2, 3 AML treatment includes at least one course of induction chemotherapy including daunorubicin (DNR) and cytarabine. 1 AML is the most common type of leukemia in adults with lowest survival rate of all leukemias. Results of apoptosis assay showed that the cytotoxic effects are related to the enhancement of apoptosis.Д¬onclusion: Our study suggests that ABT-737 synergistically enhances the cytotoxic effect of DNR in AML cell lines and therefore may be useful to overcome chemoresistance of leukemia patients.ДЄcute myeloid leukemia (AML) is an aggressive blood disorder that known with the accumulation of immature hematopoietic stem cells in bone marrow. Moreover, treatment with a mixture of two agents had more growth inhibition effect relative to the monotherapy. Surprisingly, combination treatment significantly lowered the IC50 values in a synergic manner in both cell lines.

Results: IC50 values of DNR and ABT-737 were 2.52 and 0.59 ВµM for HL-60 cells line and 1.31 and 0.80 ВµM for U937 cell line at 24 h, respectively. ELISA cell death assay was used for measurement of apoptosis.

The effects of treatments on the cell proliferation was determined by trypan blue assay.

Methods: The cytotoxic effects of DNR and ABT-737, alone or in combination were assessed using MTT assay and combination index analysis. In this study the effect of small-molecule BH3-mimetic, ABT-737, on the sensitivity of HL60 and U937 AML cell lines was investigated. Purpose: Intensive chemotherapy with daunorubicin (DNR) is associated with serious side effects in acute myeloid leukemia (AML) patients.

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